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Introduction: This study aimed to evaluate the in vitro effectiveness of N-acetylcysteine (NAC), photodynamic therapy (PDT) and NAC with supplemental PDT in optimizing the removal of bacteria from infected dentinal tubules of root canals infected with Enterococcus (E.) faecalis biofilm. Methods and Materials: Eighty human teeth were randomly divided into 5 groups (n=16) according to the intracanal medication used: saline solution (control); calcium hydroxide (CH); NAC; PDT; NAC+PDT. Ten samples from each group were prepared for microbiological culture analysis (CFU/mL) and were inoculated with E. faecalis suspension for 21 days for biofilm development; the other six samples from each group were prepared for scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM) and submitted to a 5-days contamination protocol including eight centrifugation cycles on every other day for dentinal tubules infection. For antimicrobial activity analysis by microbiological culture (CFU/mL), the root canals were contaminated with E. faecalis biofilm, instrumented and then medicated according to the experimental groups. Three samples were collected from the root canals: after 21-days of contamination, immediately after the instrumentation and 14-days after the medication according to the experimental groups. The morphology of E. faecalis biofilm on the root canal walls and bacterial cells viability were assessed by means of SEM and CLSM, respectively. One-way ANOVA and Repeated Measures ANOVA tests were used to analyze the obtained data statistically. Results: CFU/mL analysis showed that CH, NAC and NAC+PDT promoted greater antibacterial activity with statistically significant difference compared to saline solution and PDT (P<0.0001). However, saline solution and PDT were statistically similar (P>0.07). Illustrative images by SEM confirmed partially the CFU/mL results. CLSM showed that all groups were effective eliminating E. faecalis except for the saline solution group. Conclusions: Based on this in vitro study NAC was bactericidal against E. faecalis biofilms regardless PDT stimulation, presenting similar antimicrobial activity to CH.
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This case report aims to describe the management of a complex odontoma with endodontic involvement of surrounding teeth utilizing a new bioceramic cement consisting of five mineral oxides (5MO) as a retro-filling material. The patient presented for routine consultation with slight dental mobility in the antero-superior region. Upon clinical and computed tomography examination, bone rarefaction was observed in the apical region of teeth 11 and 12, external root resorption in the involved teeth and necrotic pulp of tooth 12. Root canal treatment was performed in teeth 11 and 12. Later, local surgical excision of the lesion was carried out, finding a mass with clinical features of complex odontoma, with histopathological examination of the mass confirming this diagnosis. Retro-filling of tooth 12 with 5MO was carried out. No signs and symptoms were observed over twelve-months of follow-up, with bone neoformation observed in the region. Therefore, 5MO appears to be an effective bioceramic cement that has reparative features.
Assuntos
Odontoma , Periodontite Periapical , Adulto , Feminino , Humanos , Óxidos , Tratamento do Canal RadicularRESUMO
This article presents a case of odontogenic keratocyst (OKC) located in the mandible, involving teeth 36 to 45, with significant loss of alveolar bone and aseptic pulp necrosis, emphasizing on root canal treatment after surgical intervention. Orthopantomogram and computed tomography examinations revealed an extensive, well-defined, and multilocular radiolucent lesion. Histopathological examination after incisional biopsy confirmed OKC, which was removed completely with enucleation and curettage, followed by the endodontic treatments of teeth 36 to 45 using reciprocating nickel-titanium files (Reciproc) in a single session. Afterwards, teeth 33 to 36 underwent apical surgery to create an appropriate bone development. Panoramic radiographic images showed bone formation and no sign of recurrence after one-year follow-up. In conclusion, this surgical approach, combined with the endodontic treatments of the teeth involved in the lesion, was effective for the management of OKC, promoting injury regression and preservation of the natural teeth.
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Objective: To assess the effect of simulated brushing after different treatments in color maintenance and sur-face roughness of a ceramic. Materials and methods: Thirty lithium disilicate ceramic blocks were obtained, sintered, finished with water abrasive paper grit, and divided into: Group P- polished with diamond-coated silicone tips; Group G- glaze; Group S- pigment layer and glaze. A brushing simulator machine (37 oC/120 rpm) was used (10,000; 30,000; and 100,000 cycles) and surface roughness analysis was performed before and after this simulation in a rugosimeter (Ra parame-ters - mean roughness). The samples color were assessed with a spectrophotometer (Easyshade, Vita; parameters L*a*b*). Results: Regarding surface roughness, Group P presented statistical difference after 10 years. Group G presented difference for the initial measurement, and after 1 and 3 years. Regarding color, Group P did not vary after 10 years. Group G presented difference for the initial measurement, and after 1 and 3 years; Group P did not vary after brushing, except for 1 and 10 years The initial color of Group G was similar after 1 and 3 ye-ars, however, it presented statistical difference after 10 years. Group S presented similar behavior between the initial periods from 1 to 3 years, however, the initial ?E differed statistically for 10 years. Group S were shown to be statistically different from others for all periods, ex-cept for S and P in 1 year. Conclusion: It was concluded that Group P did not suffer initial color alteration after simulated brushing, and Group G and S suffered color alteration after 10 years of simulated brushing.
